Real-time imaging of blood flow

Laser Speckle Contrast Imaging (LSCI), a less-used technique, is a real-time wide-field technology that can offer a powerful means to assess tissue blood flow in both human and animal tissues throughout a wide dynamic range (WDR). The full-field vision and high spatio­temporal resolution of LSCI is drawing interest from the scientific and medical communities. Scientists from China's Capital Medical University, Technical Institute of Physics and Chemistry, and University of Chinese Academy of Sciences have shown via animal experi­ments that the LSCI technique is capable of measuring velocities over a wide dynamic range in real-time. The imaging system documented the spatiotemporal evolution of myocardial perfusion in coronary artery bypass grafting, an advancement that could be of great benefit for future research in the life sciences and clinical medicine. All experiments were carried out in accor­dance with the guidelines for the humane care of animals. 

The experimental setup consisted of a 785 nanometer wavelength laser diode placed in a mount with a thermoelectric cooling stage. Before the samples were illuminated, a light pipe homo­genized the laser beam. The imaging system itself was composed of a Mikrotron EoSens 4CXP CoaXPress CMOS camera set to acquire images at 2336 × 1728 resolution with 563 frames per second, along with a bandpass filter, polarizer, tube lens, plus an objective lens. The Mikrotron camera's four CXP-6 channels were connected to a single CXP frame grabber via coaxial cables trans­mitting at a total of 25 Gigabit per second. Using the camera, the WDR-LSCI method was success­fully demonstrated to measure a high blood flow rate with superior per­formance when compared to TTFM. The new system showed higher sensitivity and the ability to obtain a wide-field, high-precision blood flow rate map in the non-contact way while imaging in vivo. 

SVS-Vistek is under the TKH Vision umbrella in the TKH Group. Mikro­tron EoSens CoaXPress cameras are available in either CXP6 or CXP12 interfaces in resolutions as high as 21 megapixels. Using four parallel lanes, data rates of up to 50 Gigabit per second are possible with the CXP12 designs in both monochrome and color. (Source: SVS-Vistek)

Reference: H. L. Liu et al.: Wide dynamic range measurement of blood flow in vivo using laser speckle contrast imaging, J. Biomed. Opt. 29, 016009 (2024; DOI: 10.1117/1.JBO.29.1.016009

Link: Beijing Anzhen Hospital, Capital Medical U., Beijing, China • Mikrotron, SVS-Vistek GmbH, Gilching, Germany