Olympus has introduced a new 25x water immersion objective specifically designed for multiphoton imaging. It offers researchers outstanding deep-imaging and high resolution performance with the Olympus FluoView FV1000MPE multiphoton laser-scanning microscope system. The new Olympus XLPlan N 25x objective has been designed with a numerical aperture (NA) of 1.05 for high-resolution multiphoton microscopy. It can be used for tissue samples with cover slips and for investigations, such as patch clamping, where the sample cannot be covered. As a result, higher z-resolution and significantly increased brightness is achieved for all deep imaging applications, compared to similar objectives already on the market. With a working distance of 2 mm and a large 35-degree access angle available for patch clamping, this objective is excellent for neurophysiology and related applications.