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New frequency comb identifies molecules faster

22.11.2023 - Laser-based system captures moment-to-moment details of high-speed processes such as protein folding.

From monitoring concentrations of greenhouse gases to detecting Covid in the breath, frequency combs can identify specific molecules as simple as carbon dioxide and as complex as monoclonal antibodies with unprecedented accuracy and sensitivity. However, frequency combs have been limited in how fast they can capture a high-speed process such as hypersonic propulsion or the folding of proteins into their final three-dimen­sional shapes. Now, researchers at the National Institute of Standards and Techno­logy (NIST), Toptica Photonics AG and the University of Colorado Boulder have developed a frequency comb system that can detect the presence of specific molecules in a sample every 20 nanoseconds.

With this new capability, researchers can potentially use frequency combs to better understand the split-second intermediate steps in fast-moving processes ranging from the workings of hypersonic jet engines to the chemical reactions between enzymes that regulate cell growth. In their experiment, the researchers used the now-common dual-frequency comb setup, which contains two laser beams that work together to detect the spectrum of colors that a molecule absorbs. Most dual-frequency comb setups involve two femto­second lasers, which send out a pair of ultrafast pulses in lockstep. In this new experiment, the researchers used a simpler and cheaper setup of electro-optic combs, in which a single continuous beam of light first gets split into two beams. Then, an electronic modulator produces electric fields that alter each light beam, shaping them into the individual teeth of a frequency comb. Each tooth is a specific color or frequency of light that can then be absorbed by a molecule of interest.

Whereas conventional frequency combs can have thousands or even millions of teeth, the researchers’ electro-optic comb only had 14 in a typical experi­mental run. However, as a result, each tooth had much higher optical power, and was far apart from others in frequency, resulting in a clear, strong signal that enabled the researchers to detect changes in the absorption of light at the 20-nanosecond time scale. In their demons­tration, the researchers used the instrument to measure supersonic pulses of CO2 emerging from a small nozzle in an air-filled chamber. They measured the CO2 mixing ratio, the proportion of carbon dioxide in the air. The changing concentration of CO2 told researchers about the motion of the pulse. The researchers saw how the CO2 inter­acted with the air and created oscilla­tions of air pressure in its wake. Such details are often hard to accurately obtain even with the most sophisticated computer simulations.

“In a more complicated system like an aircraft engine we could use this approach to look at a particular species of interest, such as water or fuel or CO2, to observe the chemistry. We can also use this approach to measure things such as pressure, temperature or velocity by looking at changes in the signal,” said NIST research chemist David Long. The information from these experiments could provide insights that could lead to design improvements in combustion engines, or a better under­standing of how greenhouse gases interact with the atmosphere. An optical parametric oscillator was used to shift the comb teeth from the near-infrared to the mid-infrared colors absorbed by CO2. But the optical para­metric oscillator can also be tuned to other regions of the mid-infrared so that the combs can detect other molecules that absorb light in those regions.

“What is truly special about this work is that it substantially lowers the barrier to entry for researchers who would like to use frequency combs to study fast processes,” said Greg Rieker, a professor at the University of Colorado Boulder. “With this setup, you can generate any comb you want. The tunability, flexi­bility and speed of this method open the door to lots of different types of measure­ments,” Long said. (Source: NIST)

Reference: D. A. Long et al.: Nanosecond time-resolved dual-comb absorption spectroscopy, Nat. Phot., online 30 October 2023, DOI: 10.1038/s41566-023-01316-8

Link: Optical Measurements Group, National Institute of Standards and Technology NIST, Gaithersburg, USA

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